Cellular and acellular ex vivo lung perfusion preserve functional lung ultrastructure in a large animal model: a stereological study

Ex vivo lung perfusion in Brazil

OBJECTIVE To evaluate the use of ex vivo lung perfusion (EVLP) clinically to prepare donor lungs for transplantation. METHODS A prospective study involving EVLP for the reconditioning of extended-criteria donor lungs, the criteria for which include aspects such as a PaO2/FiO2 ratio < 300 mmHg. Between February of 2013 and February of 2014, the lungs of five donors were submitted to EVLP for u...

Towards a better understanding of ex vivo lung perfusion.

Ex vivo lung perfusion (EVLP) is probably the most promising technique currently available to substantially increase the number of acceptable donor lungs and to improve the outcome after lung transplantation. Several ongoing prospective single and multicentre trials are currently investigating the potential and the individual advantages of different platforms available for EVLP. So far, a numbe...

Comparison of lung preservation solutions in human lungs using an ex vivo lung perfusion experimental model

OBJECTIVE Experimental studies on lung preservation have always been performed using animal models. We present ex vivo lung perfusion as a new model for the study of lung preservation. Using human lungs instead of animal models may bring the results of experimental studies closer to what could be expected in clinical practice. METHOD Brain-dead donors whose lungs had been declined by transpla...

New perspectives in lung transplantation: from conventional preservation to ex vivo lung perfusion and lung reconditioning.

Epigenetic: A missing paradigm in cellular and molecular pathways of sulfur mustard lung: a prospective and comparative study

Sulfur mustard (SM, bis- (2-chloroethyl) sulphide) is a chemical warfare agent that causes DNA alkylation, protein modification and membrane damage. SM can trigger several molecular pathways involved in inflammation and oxidative stress, which cause cell necrosis and apoptosis, and loss of cells integrity and function. Epigenetic regulation of gene expression is a growing research topic and is ...